446 ALOX12B and PNPLA1 have distinct roles in lamellar lipid organization

Changes in the abundance and organization of lipid lamellae (LL) stratum corneum (SC) are proposed to underly barrier dysfunction conditions such as psoriasis, atopic dermatitis ichthyosis. However, there few reports on LL diseased SC. The current investigation tested hypothesis that is altered omega-O-acylceramide metabolism defects associated with congenital Pnpla1 Alox12b knockout mice were used experimental models defective synthesis oxidation, respectively, was quantitatively evaluated by electron microscopy. Pnpla1-/- Alox12b-/- SC thicker than control contained a greater quantity mature LL. deletion, but not disorganization, loss normal pairing triplet-grouping immature LL, respectively. 19 21% thinner their corresponding Contrary previously thought, no evidence resulted from failure form or LL-poor extracellular spaces. Together, these results indicate differentially expression, while both These changes could contribute increased skin permeability ichthyosis other diseases provide insights into functions Pnpla1.